|
KMID : 0811720080120020043
|
|
Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 2 p.43 ~ p.50
|
|
|
Effects of Apigenin on Glutamate-induced [Ca2+]i Increases in Cultured Rat Hippocampal Neurons
|
|
Han Ji-Hwa
Lee Mun-Yong
Kim Ki-Jung
Hahn Sang-June
Sung Ki-Wug
Kim Myung-Jun
Yoon Shin-Hee
Jo Yang-Hyeok
Rhie Duck-Joo
Jang Hyun-Jong
Jang Ju-Ho
|
|
|
Abstract
|
|
|
Flavonoids have been shown to affect calcium signaling in neurons. However, there are no reports on the effect of apigenin on glutamate-induced calcium signaling in neurons. We investigated whether apigenin affects glutamate-induced increase of free intracellular Ca2+ concentration ([Ca2+]i) in cultured rat hippocampal neurons, using fura-2-based digital calcium imaging and microfluorimetry. The hippocampal neurons were used between 10 and 13 days in culture from embryonic day 18 rats. Pretreatment of the cells with apigenin (1μM to 100μM) for 5 min inhibited glutamate (100μM, 1
min) induced [Ca2+]i increase, concentration-dependently. Pretreatment with apigenin (30μM) for 5 min significantly decreased the [Ca2+]i responses induced by two ionotropic glutamate receptor agonists, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPA, 10μM, 1 min) and N-methyl-D-aspartate (NMDA, 100μM, 1 min), and significantly inhibited the AMPA-induced peak currents. Treatment with apigenin also significantly inhibited the [Ca2+]i response induced by 50 mM KCl solution, decreased the [Ca2+]i responses induced by the metabotropic glutamate receptor agonist, (S)-3,5-dihydroxyphenylglycine (DHPG, 100μM, 90 s), and inhibited the caffeine (10 mM, 2 min)-induced [Ca2+]i
responses. Furthermore, treatment with apigenin (30μM) significantly inhibited the amplitude and frequency of 0.1 mM [Mg2+]o-induced [Ca2+]i spikes. These data together suggest that apigenin inhibits glutamate-induced calcium signaling in cultured rat hippocampal neurons.
|
|
|
KEYWORD
|
|
|
Apigenin, Intracellular calcium, Hippocampal neuron, Glutamate, Flavonoid
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|